SEQUENCING / BIO-DREAM
Whole-genome resequencing
Plan a short-read genome project with the reference, target coverage and deliverables agreed before samples arrive. We welcome plant, animal and microbial research enquiries.
Where this fits your work
- Research comparisons across strains, individuals or populations
- Reference-based sequence variation studies
- Pilot projects before a larger sample cohort
Build a complete project brief
| Item | What to confirm |
|---|---|
| Biology | Species, estimated genome size, ploidy and reference assembly version. |
| Data target | State Gb per sample or target depth; define raw versus clean bases and paired reads versus read pairs. |
| Library | Confirm library preparation, PCR-free feasibility, read configuration and platform. |
| Analysis | Agree mapping, variant classes, filtering criteria and required reports. |
Define what you receive
- FASTQ and sequencing quality summaries as agreed.
- Mapped alignments and variant files only when contracted.
- Record the reference version and analysis scope for reproducibility.
These are quotation checklist items, not a guarantee that every listed output is included. Ask us to define the exact scope before ordering.
A local conversation. A clearly scoped project.
Share your sample count, intended output and preferred schedule. Bio-Dream coordinates the quotation and Hong Kong delivery arrangements.
Prepare a project enquiryQuestions before you begin
Does 30× mean every position has 30 reads?
No. It is an average or target measure; coverage varies by region. Define mapped depth and coverage breadth separately in the quotation.
Is resequencing the same as de novo assembly?
No. Reference-based resequencing and building a new genome assembly have different data and analysis requirements. Please flag a de novo objective at enquiry.