SEQUENCING / BIO-DREAM
RNA sequencing & transcriptomics
Build an expression study around your biological comparison, not just a read count. Define groups, replicates, RNA type and analysis objectives with your project brief.
Where this fits your work
- Compare expression between experimental conditions
- Explore treatment-response and functional pathway research
- Discuss coding-RNA or broader transcript coverage
Build a complete project brief
| Item | What to confirm |
|---|---|
| Design | Species, groups, biological replicates and known batch factors. |
| RNA input | Extraction method, concentration, purity and integrity assessment, where available. |
| Selection | Discuss poly(A) selection or rRNA depletion according to organism, RNA quality and target. |
| Sequencing scope | Confirm strand specificity, read layout, output units and analysis comparisons. |
Define what you receive
- Agree FASTQ, quality metrics and alignment summaries.
- Count or abundance tables and differential-expression outputs where included.
- Specify enrichment analysis, plots and comparison groups in advance.
These are quotation checklist items, not a guarantee that every listed output is included. Ask us to define the exact scope before ordering.
A local conversation. A clearly scoped project.
Share your sample count, intended output and preferred schedule. Bio-Dream coordinates the quotation and Hong Kong delivery arrangements.
Prepare a project enquiryQuestions before you begin
Can deeper sequencing replace biological replicates?
No. More reads and biological replication address different sources of uncertainty. Discuss both before committing the study budget.
Does RNA abundance directly measure protein activity?
No. RNA expression is one layer of evidence; protein abundance and activity may differ and may need separate assays.